From mammalian tissue engineering to 3D plant cell culture

Mammalian Cell Tissue Culture Techniques.

Cultured tissues and cells are used extensively in physiological and pharmacological studies. In vitro cultures provide a means of examining cells and tissues without the complex interactions that would be present if the whole organism were studied. A number of special skills are required in order to preserve the structure, function, behavior, and biology of cells in culture. This unit describe...

Hydrodynamic 3D Culture for Bone Tissue Engineering

Bone tissue engineering provided a promising approach for treatment of large bone defects resulting from maladies such as birth defects, trauma, or tumor resection. In vitro culture of a porous scaffold seeded with osteoprogenitor cells may enhance its bone regeneration potential. In this chapter, we describe the design of a novel perfusion bioreactor system with oscillatory flow for cultivatin...

Secondary Products from Plant Tissue Culture

Wound Healing: From Epidermis Culture to Tissue Engineering

The skin is the largest organ of the individual, being the interface between the body and the microenvironment. In severely burned patients and other diseases, the physiological processes of wound healing are not sufficient to complete the closure of their wounds. The in vitro culture of autologous epidermis, which has represented the beginning of Tissue Engineering, is a valuable tool for the ...

Apple Derived Cellulose Scaffolds for 3D Mammalian Cell Culture

There are numerous approaches for producing natural and synthetic 3D scaffolds that support the proliferation of mammalian cells. 3D scaffolds better represent the natural cellular microenvironment and have many potential applications in vitro and in vivo. Here, we demonstrate that 3D cellulose scaffolds produced by decellularizing apple hypanthium tissue can be employed for in vitro 3D culture...